{"id":1383,"date":"2013-07-13T10:02:54","date_gmt":"2013-07-13T17:02:54","guid":{"rendered":"http:\/\/kezune.com\/sim\/?p=1383"},"modified":"2014-06-28T00:01:19","modified_gmt":"2014-06-28T07:01:19","slug":"discus-splicing-part-2","status":"publish","type":"post","link":"http:\/\/kezune.com\/sim\/2013\/07\/13\/discus-splicing-part-2\/","title":{"rendered":"Discus Splicing Part 2"},"content":{"rendered":"<p style=\"text-align: center;\">Last time I spliced Discus genes, I only made it to line C before, if I&#8217;m honest, it was time for me to go to bed. Now that I&#8217;m back, it&#8217;s time to check out the other genes. I still haven&#8217;t found the genes that affect the Discus&#8217; colors.<\/p>\n<p style=\"text-align: center;\"><a href=\"http:\/\/kezune.com\/sim\/wp-content\/uploads\/2013\/07\/bandicam-2013-07-09-00-00-26-430.png\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-1370\" src=\"http:\/\/kezune.com\/sim\/wp-content\/uploads\/2013\/07\/bandicam-2013-07-09-00-00-26-430.png\" alt=\"bandicam 2013-07-09 00-00-26-430\" width=\"228\" height=\"327\" srcset=\"http:\/\/kezune.com\/sim\/wp-content\/uploads\/2013\/07\/bandicam-2013-07-09-00-00-26-430.png 228w, http:\/\/kezune.com\/sim\/wp-content\/uploads\/2013\/07\/bandicam-2013-07-09-00-00-26-430-209x300.png 209w\" sizes=\"auto, (max-width: 228px) 100vw, 228px\" \/><\/a><\/p>\n<p style=\"text-align: center;\">In case you forgot, here&#8217;s the box to show the rows I&#8217;m editing. Last time we stopped after C, today we&#8217;re finishing D through H, time allowing. Also, it turns out I goofed and miscounted the number of lines. My bad!<\/p>\n<h2 style=\"text-align: center;\">D<\/h2>\n<p style=\"text-align: center;\"><strong>D 25<\/strong><\/p>\n<p style=\"text-align: center;\"><a href=\"http:\/\/kezune.com\/sim\/wp-content\/uploads\/2013\/07\/bandicam-2013-07-09-05-24-26-355.png\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-1384\" src=\"http:\/\/kezune.com\/sim\/wp-content\/uploads\/2013\/07\/bandicam-2013-07-09-05-24-26-355.png\" alt=\"bandicam 2013-07-09 05-24-26-355\" width=\"156\" height=\"160\" \/><\/a><\/p>\n<p style=\"text-align: center;\">Yellow fish! Now we&#8217;re finally getting somewhere. Unless I&#8217;m mistaken, yellow is one of the two base colors with blue being the other. I&#8217;m only basing this on the two discus .fsh files that come with the addon pack. D 50 and D 75 produce the same result.<\/p>\n<p style=\"text-align: center;\"><strong>D 100<\/strong><\/p>\n<p style=\"text-align: center;\"><a href=\"http:\/\/kezune.com\/sim\/wp-content\/uploads\/2013\/07\/bandicam-2013-07-09-05-28-02-005.png\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-1385\" src=\"http:\/\/kezune.com\/sim\/wp-content\/uploads\/2013\/07\/bandicam-2013-07-09-05-28-02-005.png\" alt=\"bandicam 2013-07-09 05-28-02-005\" width=\"173\" height=\"174\" srcset=\"http:\/\/kezune.com\/sim\/wp-content\/uploads\/2013\/07\/bandicam-2013-07-09-05-28-02-005.png 173w, http:\/\/kezune.com\/sim\/wp-content\/uploads\/2013\/07\/bandicam-2013-07-09-05-28-02-005-150x150.png 150w\" sizes=\"auto, (max-width: 173px) 100vw, 173px\" \/><\/a><\/p>\n<p style=\"text-align: center;\">D 100 produces a much darker, more natural looking yellow. We&#8217;ll call it canary yellow. I was expecting the color to work in shades, depending on the &#8216;intensity&#8217; of this gene but that isn&#8217;t the case.<\/p>\n<p style=\"text-align: center;\">Anyway, let&#8217;s see how D affects the colors from line A.<\/p>\n<p style=\"text-align: center;\"><strong>A 50 &#8211; A 100<\/strong><br \/>\n<strong>D 50<\/strong><\/p>\n<p style=\"text-align: center;\"><a href=\"http:\/\/kezune.com\/sim\/wp-content\/uploads\/2013\/07\/bandicam-2013-07-09-05-33-21-233.png\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-1386\" src=\"http:\/\/kezune.com\/sim\/wp-content\/uploads\/2013\/07\/bandicam-2013-07-09-05-33-21-233.png\" alt=\"bandicam 2013-07-09 05-33-21-233\" width=\"321\" height=\"142\" srcset=\"http:\/\/kezune.com\/sim\/wp-content\/uploads\/2013\/07\/bandicam-2013-07-09-05-33-21-233.png 321w, http:\/\/kezune.com\/sim\/wp-content\/uploads\/2013\/07\/bandicam-2013-07-09-05-33-21-233-300x132.png 300w\" sizes=\"auto, (max-width: 321px) 100vw, 321px\" \/><\/a><\/p>\n<p style=\"text-align: center;\">Changing D doesn&#8217;t seem to affect the color of the stripes at all. Oh well.<\/p>\n<p style=\"text-align: center;\">B only affects the intensity of the stripes and C affects the intensity of vertical bars.<\/p>\n<h2 style=\"text-align: center;\">E<\/h2>\n<p style=\"text-align: center;\"><strong>E 25 &#8211; 75<\/strong><\/p>\n<p style=\"text-align: center;\"><a href=\"http:\/\/kezune.com\/sim\/wp-content\/uploads\/2013\/07\/bandicam-2013-07-09-05-39-10-746.png\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-1387\" src=\"http:\/\/kezune.com\/sim\/wp-content\/uploads\/2013\/07\/bandicam-2013-07-09-05-39-10-746.png\" alt=\"bandicam 2013-07-09 05-39-10-746\" width=\"154\" height=\"150\" \/><\/a><\/p>\n<p style=\"text-align: center;\">Ooh! Red seems like another base color. As with yellow, values 25, 50 and 75 produce the result above.<\/p>\n<p style=\"text-align: center;\"><strong>E 100<\/strong><\/p>\n<p style=\"text-align: center;\"><a href=\"http:\/\/kezune.com\/sim\/wp-content\/uploads\/2013\/07\/bandicam-2013-07-09-05-41-10-392.png\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-1388\" src=\"http:\/\/kezune.com\/sim\/wp-content\/uploads\/2013\/07\/bandicam-2013-07-09-05-41-10-392.png\" alt=\"bandicam 2013-07-09 05-41-10-392\" width=\"150\" height=\"142\" \/><\/a><\/p>\n<p style=\"text-align: center;\">E 100 produces this gorgeous shade of red. Knowing what we do now about this gene, let&#8217;s see how it interacts with other genes.<\/p>\n<p style=\"text-align: center;\"><strong>A 50 &#8211; A 100<\/strong><br \/>\n<strong> E 100<\/strong><\/p>\n<p style=\"text-align: center;\"><a href=\"http:\/\/kezune.com\/sim\/wp-content\/uploads\/2013\/07\/bandicam-2013-07-09-05-44-45-235.png\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-1389\" src=\"http:\/\/kezune.com\/sim\/wp-content\/uploads\/2013\/07\/bandicam-2013-07-09-05-44-45-235.png\" alt=\"bandicam 2013-07-09 05-44-45-235\" width=\"316\" height=\"150\" srcset=\"http:\/\/kezune.com\/sim\/wp-content\/uploads\/2013\/07\/bandicam-2013-07-09-05-44-45-235.png 316w, http:\/\/kezune.com\/sim\/wp-content\/uploads\/2013\/07\/bandicam-2013-07-09-05-44-45-235-300x142.png 300w\" sizes=\"auto, (max-width: 316px) 100vw, 316px\" \/><\/a><\/p>\n<p style=\"text-align: center;\">As above, B only affects the intensity of the stripes and C affects the intensity of vertical bars.<\/p>\n<p style=\"text-align: center;\"><strong>D 50 &#8211; E 50<\/strong><a href=\"http:\/\/kezune.com\/sim\/wp-content\/uploads\/2013\/07\/bandicam-2013-07-09-05-50-03-779.png\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-1390\" src=\"http:\/\/kezune.com\/sim\/wp-content\/uploads\/2013\/07\/bandicam-2013-07-09-05-50-03-779.png\" alt=\"bandicam 2013-07-09 05-50-03-779\" width=\"158\" height=\"150\" \/><\/a><\/p>\n<p style=\"text-align: center;\">When I mix our two color genes, something very interesting happens. The colors blend! This is a gorgeous shade on its own already but, unfortunately, this is the only color we can get by mixing these genes. I don&#8217;t just mean orange, I mean this particular intensity is all we can get.<\/p>\n<h2 style=\"text-align: center;\">F<\/h2>\n<p style=\"text-align: center;\"><strong>F 50<\/strong><\/p>\n<p style=\"text-align: center;\"><a href=\"http:\/\/kezune.com\/sim\/wp-content\/uploads\/2013\/07\/bandicam-2013-07-09-05-54-38-335.png\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-1391\" src=\"http:\/\/kezune.com\/sim\/wp-content\/uploads\/2013\/07\/bandicam-2013-07-09-05-54-38-335.png\" alt=\"bandicam 2013-07-09 05-54-38-335\" width=\"153\" height=\"152\" srcset=\"http:\/\/kezune.com\/sim\/wp-content\/uploads\/2013\/07\/bandicam-2013-07-09-05-54-38-335.png 153w, http:\/\/kezune.com\/sim\/wp-content\/uploads\/2013\/07\/bandicam-2013-07-09-05-54-38-335-150x150.png 150w\" sizes=\"auto, (max-width: 153px) 100vw, 153px\" \/><\/a><\/p>\n<p style=\"text-align: center;\">Ooh! This is a neat color. Of all the colors out there, grey was probably the last I expected to see. Let&#8217;s see what happens when I change the value to 100.<\/p>\n<p style=\"text-align: center;\"><strong>F 100<\/strong><\/p>\n<p style=\"text-align: center;\"><a href=\"http:\/\/kezune.com\/sim\/wp-content\/uploads\/2013\/07\/bandicam-2013-07-09-05-55-29-441.png\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-1392\" src=\"http:\/\/kezune.com\/sim\/wp-content\/uploads\/2013\/07\/bandicam-2013-07-09-05-55-29-441.png\" alt=\"bandicam 2013-07-09 05-55-29-441\" width=\"148\" height=\"143\" \/><\/a><\/p>\n<p style=\"text-align: center;\">Black! We have a black discus now. Now that we know what this does to the fish&#8217;s base, let&#8217;s start mixing it with other genes.<\/p>\n<p style=\"text-align: center;\"><a href=\"http:\/\/kezune.com\/sim\/wp-content\/uploads\/2013\/07\/bandicam-2013-07-09-06-07-14-201.png\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-1393\" src=\"http:\/\/kezune.com\/sim\/wp-content\/uploads\/2013\/07\/bandicam-2013-07-09-06-07-14-201.png\" alt=\"bandicam 2013-07-09 06-07-14-201\" width=\"731\" height=\"141\" srcset=\"http:\/\/kezune.com\/sim\/wp-content\/uploads\/2013\/07\/bandicam-2013-07-09-06-07-14-201.png 731w, http:\/\/kezune.com\/sim\/wp-content\/uploads\/2013\/07\/bandicam-2013-07-09-06-07-14-201-300x57.png 300w\" sizes=\"auto, (max-width: 731px) 100vw, 731px\" \/><\/a><\/p>\n<p style=\"text-align: center;\">All have F set to 50. In order from left to right.<br \/>\nA 50 &#8211; C 50 &#8211; D 50 &#8211; E 50 &#8211; D 50 E 50<\/p>\n<p style=\"text-align: center;\">Such pretty fish so far! But I&#8217;m still trying to figure out what changes the stripe color and eye color. Since I don&#8217;t know yet, let&#8217;s press on.<\/p>\n<h2 style=\"text-align: center;\">G<\/h2>\n<p style=\"text-align: center;\"><strong>G 50<\/strong><\/p>\n<p style=\"text-align: center;\"><a href=\"http:\/\/kezune.com\/sim\/wp-content\/uploads\/2013\/07\/bandicam-2013-07-09-06-11-00-766.png\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-1394\" src=\"http:\/\/kezune.com\/sim\/wp-content\/uploads\/2013\/07\/bandicam-2013-07-09-06-11-00-766.png\" alt=\"bandicam 2013-07-09 06-11-00-766\" width=\"149\" height=\"146\" \/><\/a><\/p>\n<p style=\"text-align: center;\">Do you remember what I was saying in line C where C and G work together to form bars on the fish? Turns out I misread the site. That&#8217;s not til the next line.<\/p>\n<p style=\"text-align: center;\">I edited the other genes we&#8217;ve tested and I still come up with no changes to the fish at all, not even to the color of the stripes, bars or eye of the fish. Frustrated, I did some additional research.<\/p>\n<p style=\"text-align: center;\"><a href=\"http:\/\/kezune.com\/sim\/wp-content\/uploads\/2013\/07\/bandicam-2013-07-09-06-34-43-894.png\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-1395\" src=\"http:\/\/kezune.com\/sim\/wp-content\/uploads\/2013\/07\/bandicam-2013-07-09-06-34-43-894.png\" alt=\"bandicam 2013-07-09 06-34-43-894\" width=\"161\" height=\"155\" \/><\/a><\/p>\n<p style=\"text-align: center;\">As it turns out, A and B set to 100produce a blue-green discus.<\/p>\n<p style=\"text-align: center;\"><a href=\"http:\/\/kezune.com\/sim\/wp-content\/uploads\/2013\/07\/bandicam-2013-07-09-06-38-51-365.png\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-1396\" src=\"http:\/\/kezune.com\/sim\/wp-content\/uploads\/2013\/07\/bandicam-2013-07-09-06-38-51-365.png\" alt=\"bandicam 2013-07-09 06-38-51-365\" width=\"158\" height=\"152\" \/><\/a><\/p>\n<p style=\"text-align: center;\">Changing G to 50 produces a darker blue. It&#8217;s difficult for me to agree with the game&#8217;s assessment that blue is a base color since it seems like blue acts more like an overlay color. Maybe they&#8217;re referring to the overlay color in the file names. Still, red and yellow are probably more fitting choices though it&#8217;s difficult to say that as well since the colors blend and work together. Changing G again to 100 produces the blue-green fish above. Changing A to 50 produces an albino fish with a Halo in dark blue instead of blue-green. Someday we&#8217;ll crack this. I found discus online with gold halos and I don&#8217;t seem to be able to produce that in FisherMan. I&#8217;m stubborn though!<\/p>\n<p style=\"text-align: center;\">After opening the Gene window in FisherMan, I found that there was a gene that I could not edit. Where my discus with a blue halo had a value of 1 for that gene, the discus I imported with golden halos had a value of 0. This gene, naturally is the second part of H. Even after going through each gene and chaning the values to 1-16, the 16th gene changes back to 1. 1 seems to be dominant.<\/p>\n<p style=\"text-align: center;\">Eventually, I loaded up a hex editor (in my case HxD, though ResHack works, too) and scrolled down to the bottom. To make it easy for me to spot the offending gene, I spliced some fish to have the numbers 1 through 16 as their genotype. Naturally, the 16th gene stayed 1. I scrolled down looking for the number sequence and voila!<\/p>\n<p style=\"text-align: center;\"><a href=\"http:\/\/kezune.com\/sim\/wp-content\/uploads\/2013\/07\/bandicam-2013-07-09-08-38-04-419.png\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-1397\" src=\"http:\/\/kezune.com\/sim\/wp-content\/uploads\/2013\/07\/bandicam-2013-07-09-08-38-04-419.png\" alt=\"bandicam 2013-07-09 08-38-04-419\" width=\"611\" height=\"69\" srcset=\"http:\/\/kezune.com\/sim\/wp-content\/uploads\/2013\/07\/bandicam-2013-07-09-08-38-04-419.png 611w, http:\/\/kezune.com\/sim\/wp-content\/uploads\/2013\/07\/bandicam-2013-07-09-08-38-04-419-300x33.png 300w\" sizes=\"auto, (max-width: 611px) 100vw, 611px\" \/><\/a><\/p>\n<p style=\"text-align: center;\">There it was, the solution to my problem. The value was originally 01. I changed both fish so that the value was 0 and out came these lovelies (after some edits).<\/p>\n<p style=\"text-align: center;\"><a href=\"http:\/\/kezune.com\/sim\/wp-content\/uploads\/2013\/07\/bandicam-2013-07-09-08-44-32-565.png\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-1398\" src=\"http:\/\/kezune.com\/sim\/wp-content\/uploads\/2013\/07\/bandicam-2013-07-09-08-44-32-565.png\" alt=\"bandicam 2013-07-09 08-44-32-565\" width=\"154\" height=\"153\" srcset=\"http:\/\/kezune.com\/sim\/wp-content\/uploads\/2013\/07\/bandicam-2013-07-09-08-44-32-565.png 154w, http:\/\/kezune.com\/sim\/wp-content\/uploads\/2013\/07\/bandicam-2013-07-09-08-44-32-565-150x150.png 150w\" sizes=\"auto, (max-width: 154px) 100vw, 154px\" \/><\/a><\/p>\n<p style=\"text-align: center;\">Isn&#8217;t that a lovely looking fish? How about we try to get gold? Above, G is set to 0. Let&#8217;s change it to 110 and see what happens.<\/p>\n<p style=\"text-align: center;\"><a href=\"http:\/\/kezune.com\/sim\/wp-content\/uploads\/2013\/07\/bandicam-2013-07-09-08-53-59-826.png\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-1399\" src=\"http:\/\/kezune.com\/sim\/wp-content\/uploads\/2013\/07\/bandicam-2013-07-09-08-53-59-826.png\" alt=\"bandicam 2013-07-09 08-53-59-826\" width=\"154\" height=\"145\" \/><\/a><\/p>\n<p style=\"text-align: center;\">Bam! G also seems to control eye color. It might be fair to call G an accent color modifier.<\/p>\n<h2 style=\"text-align: center;\">H<\/h2>\n<p style=\"text-align: center;\">As I&#8217;d suspected, changing H by itself doesn&#8217;t actually change the appearance of the fish. Instead, it&#8217;s a modifier for the stripes and the vertical bars on the fish. If set to 0, it allows Gold and White stripes. If set to 4 it allows bars to appear on the fish in different intensities than one bar or full bars.<\/p>\n<p style=\"text-align: center;\">We already know that when you set C to 0, no bars appear on the fish. When you set it to 50 with H set to 1 or 0, the fish gets one bar over its eye.<\/p>\n<p style=\"text-align: center;\"><a href=\"http:\/\/kezune.com\/sim\/wp-content\/uploads\/2013\/07\/bandicam-2013-07-09-01-15-23-0481.png\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-1401\" src=\"http:\/\/kezune.com\/sim\/wp-content\/uploads\/2013\/07\/bandicam-2013-07-09-01-15-23-0481.png\" alt=\"bandicam 2013-07-09 01-15-23-048\" width=\"726\" height=\"139\" srcset=\"http:\/\/kezune.com\/sim\/wp-content\/uploads\/2013\/07\/bandicam-2013-07-09-01-15-23-0481.png 726w, http:\/\/kezune.com\/sim\/wp-content\/uploads\/2013\/07\/bandicam-2013-07-09-01-15-23-0481-300x57.png 300w\" sizes=\"auto, (max-width: 726px) 100vw, 726px\" \/><\/a><\/p>\n<p style=\"text-align: center;\">In order from left to right, here are the combinations I used.<br \/>\nC 0 &#8211; C 50 H 1 &#8211; C 50 H 4 &#8211; C 80 H 4 &#8211; C 100<\/p>\n<ul class=\"ssb_list_wrapper\"><li class=\"fb2\" style=\"width:135px\"><iframe src=\"\/\/www.facebook.com\/plugins\/like.php?href=http%3A%2F%2Fkezune.com%2Fsim%2F2013%2F07%2F13%2Fdiscus-splicing-part-2%2F&amp;layout=button_count&amp;action=like&amp;show_faces=false&amp;share=true&amp;width=135&amp;height=21&amp;appId=307091639398582\" scrolling=\"no\" frameborder=\"0\" style=\"border:none; overflow:hidden;  width:150px; height:21px;\" allowTransparency=\"true\"><\/iframe><\/li><li class=\"twtr\" style=\"width:90px\"><a href=\"https:\/\/twitter.com\/share\" class=\"twitter-share-button\" data-url=\"http:\/\/kezune.com\/sim\/2013\/07\/13\/discus-splicing-part-2\/\">&nbsp;<\/a><script>!function(d,s,id){var js,fjs=d.getElementsByTagName(s)[0],p=\/^http:\/.test(d.location)?'http':'https';if(!d.getElementById(id)){js=d.createElement(s);js.id=id;js.src=p+':\/\/platform.twitter.com\/widgets.js';fjs.parentNode.insertBefore(js,fjs);}}(document, 'script', 'twitter-wjs');<\/script><\/li><li class=\"gplus\" style=\"width:68px\"><div class=\"g-plusone\" data-size=\"medium\" data-href=\"http:\/\/kezune.com\/sim\/2013\/07\/13\/discus-splicing-part-2\/\"><\/div><\/li><li class=\"ssb_pin\" style=\"width:47px\"><a href=\"\/\/www.pinterest.com\/pin\/create\/button\/\" data-pin-do=\"buttonBookmark\" ><img decoding=\"async\" src=\"\/\/assets.pinterest.com\/images\/pidgets\/pinit_fg_en_rect_gray_20.png\" \/><\/a><\/li><li class=\"ssb_stum\" style=\"width:75px\"><su:badge layout=\"1\" location=\"http:\/\/kezune.com\/sim\/2013\/07\/13\/discus-splicing-part-2\/\"><\/su:badge><script type=\"text\/javascript\">\r\n\t\t(function() {\r\n\t\t\tvar li = document.createElement('script'); li.type = 'text\/javascript'; li.async = true;\r\n\t\t\tli.src = ('https:' == document.location.protocol ? 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Now that I&#8217;m back, it&#8217;s time to check out the other genes. I still haven&#8217;t found the genes that affect the Discus&#8217; colors. 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