bandicam 2013-07-18 07-24-36-307Just like real discus, these two caring parents have stuck by the eggs since they were laid and are staying by them now while the fry grow up. Only 7 of our 24 eggs have hatched. 5 are female, 2 are male. Isn’t this exciting? It’ll be a while yet til we can see what the babies look like but we’re well on our way.

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Holy WOW, everyone! My discus have finally bred and made babies. This time, it’s Dibella and Spartacus! I can’t seem to open up their Genes for viewing so let’s see if we can at least estimate their phenotypes. Naturally bred fish don’t operate on degrees of 25 for most of their genes like I used in my gene study but we’ll be using them to guess anyway.

There are a pair of genes for each line (A-H) but we’re going to base this strictly on their phenotype. Maybe we’ll learn something about gene dominance in these discus when the babies are born!

bandicam 2013-07-15 17-05-17-952 A – 50
B – 50
C – 0
D – 50
E – 0
F – 100
G – 100
H – 0
Dark Red Discus
Thick Blue Halo
No Bars
bandicam 2013-07-15 17-05-36-531 A – 100
B – 0
C – 50
D – 50
E – 50
F – 0
G – 0
H – 0
Orange Discus
Blue-Green Stripes
One Bar

My eyes could very well be fooling me but that’s what I see. I wish I could double check but, unfortunately, FisherMan isn’t working properly. We’ll see what the babies have to say about their parents genes when they’re born!

As of the 17th of July, their parents are still protecting their small clutch of 24 eggs.

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Last time I spliced Discus genes, I only made it to line C before, if I’m honest, it was time for me to go to bed. Now that I’m back, it’s time to check out the other genes. I still haven’t found the genes that affect the Discus’ colors.

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In case you forgot, here’s the box to show the rows I’m editing. Last time we stopped after C, today we’re finishing D through H, time allowing. Also, it turns out I goofed and miscounted the number of lines. My bad!

D

D 25

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Yellow fish! Now we’re finally getting somewhere. Unless I’m mistaken, yellow is one of the two base colors with blue being the other. I’m only basing this on the two discus .fsh files that come with the addon pack. D 50 and D 75 produce the same result.

D 100

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D 100 produces a much darker, more natural looking yellow. We’ll call it canary yellow. I was expecting the color to work in shades, depending on the ‘intensity’ of this gene but that isn’t the case.

Anyway, let’s see how D affects the colors from line A.

A 50 – A 100
D 50

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Changing D doesn’t seem to affect the color of the stripes at all. Oh well.

B only affects the intensity of the stripes and C affects the intensity of vertical bars.

E

E 25 – 75

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Ooh! Red seems like another base color. As with yellow, values 25, 50 and 75 produce the result above.

E 100

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E 100 produces this gorgeous shade of red. Knowing what we do now about this gene, let’s see how it interacts with other genes.

A 50 – A 100
E 100

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As above, B only affects the intensity of the stripes and C affects the intensity of vertical bars.

D 50 – E 50bandicam 2013-07-09 05-50-03-779

When I mix our two color genes, something very interesting happens. The colors blend! This is a gorgeous shade on its own already but, unfortunately, this is the only color we can get by mixing these genes. I don’t just mean orange, I mean this particular intensity is all we can get.

F

F 50

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Ooh! This is a neat color. Of all the colors out there, grey was probably the last I expected to see. Let’s see what happens when I change the value to 100.

F 100

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Black! We have a black discus now. Now that we know what this does to the fish’s base, let’s start mixing it with other genes.

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All have F set to 50. In order from left to right.
A 50 – C 50 – D 50 – E 50 – D 50 E 50

Such pretty fish so far! But I’m still trying to figure out what changes the stripe color and eye color. Since I don’t know yet, let’s press on.

G

G 50

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Do you remember what I was saying in line C where C and G work together to form bars on the fish? Turns out I misread the site. That’s not til the next line.

I edited the other genes we’ve tested and I still come up with no changes to the fish at all, not even to the color of the stripes, bars or eye of the fish. Frustrated, I did some additional research.

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As it turns out, A and B set to 100produce a blue-green discus.

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Changing G to 50 produces a darker blue. It’s difficult for me to agree with the game’s assessment that blue is a base color since it seems like blue acts more like an overlay color. Maybe they’re referring to the overlay color in the file names. Still, red and yellow are probably more fitting choices though it’s difficult to say that as well since the colors blend and work together. Changing G again to 100 produces the blue-green fish above. Changing A to 50 produces an albino fish with a Halo in dark blue instead of blue-green. Someday we’ll crack this. I found discus online with gold halos and I don’t seem to be able to produce that in FisherMan. I’m stubborn though!

After opening the Gene window in FisherMan, I found that there was a gene that I could not edit. Where my discus with a blue halo had a value of 1 for that gene, the discus I imported with golden halos had a value of 0. This gene, naturally is the second part of H. Even after going through each gene and chaning the values to 1-16, the 16th gene changes back to 1. 1 seems to be dominant.

Eventually, I loaded up a hex editor (in my case HxD, though ResHack works, too) and scrolled down to the bottom. To make it easy for me to spot the offending gene, I spliced some fish to have the numbers 1 through 16 as their genotype. Naturally, the 16th gene stayed 1. I scrolled down looking for the number sequence and voila!

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There it was, the solution to my problem. The value was originally 01. I changed both fish so that the value was 0 and out came these lovelies (after some edits).

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Isn’t that a lovely looking fish? How about we try to get gold? Above, G is set to 0. Let’s change it to 110 and see what happens.

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Bam! G also seems to control eye color. It might be fair to call G an accent color modifier.

H

As I’d suspected, changing H by itself doesn’t actually change the appearance of the fish. Instead, it’s a modifier for the stripes and the vertical bars on the fish. If set to 0, it allows Gold and White stripes. If set to 4 it allows bars to appear on the fish in different intensities than one bar or full bars.

We already know that when you set C to 0, no bars appear on the fish. When you set it to 50 with H set to 1 or 0, the fish gets one bar over its eye.

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In order from left to right, here are the combinations I used.
C 0 – C 50 H 1 – C 50 H 4 – C 80 H 4 – C 100

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Hey! I know this is a little unusual but I’m going to crack open FisherMan, a third-party fish and tank editor for Aquazone. What’s going to happen? It’s really anybody’s guess.

Anyway. I took my existing discus tank and decided to make a duplicate to play with today. I opened up Fish tank, hit the Splicing button and changed all of the values to 1.

bandicam 2013-07-09 00-00-26-430I realize it looks like I’ve only labelled the first row but think of the genes this way. Discus are diploid organisms. This means that they’re allowed to have dominant and recessive genes. If I make them completely homozygous, it’s easier to tell which genes produce which phenotype.

bandicam 2013-07-09 00-26-33-551Changing all of the values (A-G) to 1 produces Albino fish. I still have no idea what lines do what, but if the discus genes are anything like the guppies, the way their colors come out will depend on some sort of base gene. For now, I’m going to go down the lines and see what happens when I change each line.

A

A 50

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Changing line A seems to give the fish a striped halo. The lines are in light blue. There are no other changes to the fish. Trying the middle ground (25) between a naked fish and this halo doesn’t produce anything. So far, we’ve seen this pattern in Spartacus

A 100 (max)

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Here, we have a fish totally covered in stripes. Changing the value doesn’t seem to affect the color at all, just the intensity of the fish’s stripes. I’ve seen this particular pattern in my two female discus.

Now, let’s take the stripes off of these fish and see what happens when I change the values of the next line.

B

B 1-100

The fish doesn’t look any different from the Albino fish above. This got me curious about the relationship between B and A and if there was one.

A 50 – B 50

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Changing B to 50 seems to produce thicker stripes! Changing the value to 100 has the same result. What about when we change A to 100 for full stripes?

A 100 – B 50

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Seeing this has revealed quite a bit to me. It might look, to some, like the base color would be where the light blue is and the white would be accents, but after learning what I have so far, you could consider the base color to be covered by the stripes. Changing B to 100 produces the same result.

So far, we’ve learned that A affects striping and B affects the strength or intensity of those stripes.

Let’s move on to C.

C

C 50

bandicam 2013-07-09 01-15-23-048

Here’s where past research comes in handy for me. Once I learned that line C controlled the vertical bars on these fish, I rembered a page I read on the matter. This line and G control the intensity of the bars but it takes a value of 50 before C produces a single full bar. If you would like to read that page, you’ll find it here. We’ll learn more about C and G once we reach G.

C 100

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To my surprise, C100 gave me full bars! I have a theory now about how G affects the intensity of C now. I’m guessing that C 50 is the base for G and values G 50 and G 100 affect the intensity of C where C 100 throws full bars regardless of G. We’ll test that theory once we reach that line, though.

Learning what I can about three lines of Discus’ genetic code is enough for me for now and I feel I need to save some for next time anyway. See you with the rest soon!

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